Regulatory

Part:BBa_K613004

Designed by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer   Group: iGEM11_EPF-Lausanne   (2011-09-20)

T7 promoter family member

Parts K613001 through K613006 are T7 promoter variants. K613001 is the T7 promoter wildtype. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.

See also parts K613007 through K613012 for the same T7 promoter variants with a lac operator to add LacI repression.


T7variants.png

For more characterization data see our [http://2011.igem.org/Team:EPF-Lausanne/Our_Project/T7_promoter_variants/t7prom wiki].

1. Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419–10430 (2000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49


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Parameters
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